The Laboratory of Molecular Breeding at China Agricultural University (CAU) has achieved fruitful results in the field of genetic improvement of rice, wheat and other crops. In the extraction of nucleic acids, which is a key part of molecular breeding, traditional manual operation limits the sample throughput. The large number of samples processed daily makes manual pipetting not only inefficient, but also prone to fatigue and operational errors.
PRCXI BioWorkstation Full Process Empowerment:
In order to break through these bottlenecks and improve the efficiency and stability of experiments, China Agricultural University (CAU) has purchased PRCXI SC9000 (manual) and SC9200/SC9300 (fully automatic) pipetting workstations after many investigations and comparisons. After the first use and data verification in a laboratory building, with the significant advantages of each laboratory to recommend each other, has been the accumulated introduction of nearly 20 sets of PRCXI pipetting workstations.
Overview of the process for this type of experiment:
(Note: Due to the confidentiality of the content of the customer's experiment, this experimental process is summarized according to the type of experiment, not the actual process of the customer, and is only for the reference of customers of the same type)
1
Equipment Preparation:
PRCXI pipetting workstation, 96-well plate, tips, magnetic rack, PCR plates, reservoirs
2
Experimental Procedure:
① Add 100 μL of sample and 100 μL of lysis buffer to each well of the 96-well plate, and mix thoroughly by pipetting up and down 5 times using the workstation.
② Vortex the magnetic bead suspension thoroughly before use, add 200 μL to each reaction well, and mix by pipetting up and down 3 times using the workstation.
③ Incubate for at least 2 minutes to allow complete binding of the magnetic beads to nucleic acids.
④ Place the 96-well plate on a magnetic stand, let stand for 1 minute, then aspirate and discard the supernatant while on the stand.
⑤ Remove the plate from the magnetic stand, add wash buffer to each well, mix by pipetting up and down 5 times, and incubate for 2 minutes.
⑥ Place the plate back on the magnetic stand, let stand for 1 minute, and discard the supernatant.
⑦ Remove the plate from the magnetic stand, add wash buffer to each well, and mix by pipetting up and down 5 times.
⑧ Place the plate on the magnetic stand, let stand for 1 minute, and discard the supernatant.
⑨ Add wash buffer to each reaction well, mix by pipetting up and down 3 times, and incubate for 1 minute.
⑩ Transfer the plate to the magnetic stand, allow it to settle for 1 minute, then transfer the supernatant to a clean PCR plate (the supernatant contains the nucleic acids).
3
Experimental principle:
This product utilizes a grinding homogenizer or cell disruptor to break down plant cell walls, releasing nucleic acids (DNA or RNA). Under the lysis buffer conditions, nucleic acids specifically bind to the surface of magnetic beads. Subsequent washing steps remove impurities such as proteins and polysaccharides. Finally, the nucleic acids are eluted from the magnetic beads using an elution buffer. The purified nucleic acids can be directly used for downstream applications, including PCR detection, gene sequencing, and more.
Customer Feedback
The pipetting workstation from PRCXI has significantly improved the efficiency of the laboratory after it was put into use. The high precision of the equipment, whether it is pipetting volume or pipetting height control, has effectively avoided the loss of samples and cross-contamination, and greatly improved the stability and reliability of the experimental results.
Nowadays, the nucleic acid extraction work in the laboratory has become more efficient and accurate, which provides strong support for the further advancement of crop genetic improvement research.
